Membrane-bound SIV envelope trimers are immunogenic in ferrets after intranasal vaccination with a replication-competent canine distemper virus vector. Academic Article uri icon

Overview

MeSH

  • Administration, Intranasal
  • Animals
  • Antibodies, Viral
  • Ferrets
  • Gastrointestinal Tract
  • Lymphoid Tissue
  • Vaccines, Attenuated

MeSH Major

  • Distemper Virus, Canine
  • Drug Carriers
  • SAIDS Vaccines
  • Simian immunodeficiency virus
  • Vaccination
  • Viral Envelope Proteins

abstract

  • We are investigating canine distemper virus (CDV) as a vaccine vector for the delivery of HIV envelope (Env) that closely resembles the native trimeric spike. We selected CDV because it will promote vaccine delivery to lymphoid tissues, and because human exposure is infrequent, reducing potential effects of pre-existing immunity. Using SIV Env as a model, we tested a number of vector and gene insert designs. Vectors containing a gene inserted between the CDV H and L genes, which encoded Env lacking most of its cytoplasmic tail, propagated efficiently in Vero cells, expressed the immunogen on the cell surface, and incorporated the SIV glycoprotein into progeny virus particles. When ferrets were vaccinated intranasally, there were no signs of distress, vector replication was observed in the gut-associated lymphoid tissues, and the animals produced anti-SIV Env antibodies. These data show that live CDV-SIV Env vectors can safely induce anti-Env immune responses following intranasal vaccination. © 2013 Elsevier Inc. All rights reserved.

publication date

  • November 2013

has subject area

  • Administration, Intranasal
  • Animals
  • Antibodies, Viral
  • Distemper Virus, Canine
  • Drug Carriers
  • Ferrets
  • Gastrointestinal Tract
  • Lymphoid Tissue
  • SAIDS Vaccines
  • Simian immunodeficiency virus
  • Vaccination
  • Vaccines, Attenuated
  • Viral Envelope Proteins

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2013.07.012

PubMed ID

  • 24074564

Additional Document Info

start page

  • 25

end page

  • 36

volume

  • 446

number

  • 1-2