Cancer cell assays by use of immunocapture, subcellular imaging, and programmed cell release in gedi microdevices Conference Paper uri icon

Overview

MeSH Major

  • Microfluidic Analytical Techniques
  • Neoplastic Cells, Circulating
  • Prostatic Neoplasms

abstract

  • We report the use of Geometrically Enhanced Differential Immunocapture (GEDI) ╬╝devices, which employ staggered obstacle arrays functionalized with a prostate-specific antibody, to capture and analyze prostate cancer cells on chip. GE-DI ╬╝devices have been demonstrated to have high capture efficiency and purity [2] as compared similar devices found in the literature [4]. GEDI ╬╝devices have been used to isolate cells from cancer patient samples and are used herein to carry out in situ chemotherapeutic efficacy experiments with a model cell line, simulating a point-of-care diagnostics situation. In addition, we also demonstrate release of captured prostate cancer cells using desthiobiotin and standard antibody conjugation chemistry.

publication date

  • December 2010

Research

keywords

  • Conference Paper

Additional Document Info

start page

  • 1415

end page

  • 1417

volume

  • 2