Cancer cell assays by use of immunocapture, subcellular imaging, and programmed cell release in gedi microdevices
Microfluidic Analytical Techniques
Neoplastic Cells, Circulating
We report the use of Geometrically Enhanced Differential Immunocapture (GEDI) μdevices, which employ staggered obstacle arrays functionalized with a prostate-specific antibody, to capture and analyze prostate cancer cells on chip. GE-DI μdevices have been demonstrated to have high capture efficiency and purity  as compared similar devices found in the literature . GEDI μdevices have been used to isolate cells from cancer patient samples and are used herein to carry out in situ chemotherapeutic efficacy experiments with a model cell line, simulating a point-of-care diagnostics situation. In addition, we also demonstrate release of captured prostate cancer cells using desthiobiotin and standard antibody conjugation chemistry.