Parkin and synphilin-1 isoform expression changes in Lewy body diseases. Academic Article Article uri icon

Overview

MeSH

  • Aged
  • Aged, 80 and over
  • Alternative Splicing
  • Brain
  • Brain Chemistry
  • Disease Progression
  • Female
  • Gene Expression Regulation
  • Genetic Markers
  • Humans
  • Male
  • Middle Aged
  • Protein Isoforms
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction

MeSH Major

  • Alzheimer Disease
  • Carrier Proteins
  • Genetic Predisposition to Disease
  • Lewy Body Disease
  • Nerve Tissue Proteins
  • Parkinson Disease
  • Ubiquitin-Protein Ligases

abstract

  • Alternative splicing gives rise to at least seven parkin and eight synphilin-1 isoforms. Since both parkin and synphilin-1 have been involved in Lewy body (LB) formation, we decided to explore whether their isoforms are differentially expressed in LB diseases. With this aim, we studied relative mRNA expression levels of parkin and synphilin-1 isoforms in the frontal cortices of patients with dementia with LBs, the LB variant of Alzheimer's disease and Parkinson's disease and compared the findings with those obtained from Alzheimer's disease patients and control individuals. Duplex real-time PCR reactions, with beta-actin as internal standard, were carried out in a LightCycler. mRNA expression levels of parkin and synphilin-1 isoforms were seen to be specifically altered in each of the LB diseases studied. These findings suggest that parkin and synphilin-1 isoform expression changes play a significant role in the pathogenesis of LB diseases.

publication date

  • June 2007

has subject area

  • Aged
  • Aged, 80 and over
  • Alternative Splicing
  • Alzheimer Disease
  • Brain
  • Brain Chemistry
  • Carrier Proteins
  • Disease Progression
  • Female
  • Gene Expression Regulation
  • Genetic Markers
  • Genetic Predisposition to Disease
  • Humans
  • Lewy Body Disease
  • Male
  • Middle Aged
  • Nerve Tissue Proteins
  • Parkinson Disease
  • Protein Isoforms
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ubiquitin-Protein Ligases

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.nbd.2007.03.007

PubMed ID

  • 17467279

Additional Document Info

start page

  • 681

end page

  • 687

volume

  • 26

number

  • 3