Functional assays of drug-target engagement on circulating tumor cells captured from patient blood correlate with patient progression
Microfluidic Analytical Techniques
Neoplastic Cells, Circulating
Chemotherapeutic treatment is limited by the heterogeneous and short-lived efficacy of therapy. Functional assays of tu-bulin bundling on microchip-captured circulating tumor cells (CTCs) in late-stage castrate-resistance prostate cancer are presented for the first time, and results are interpreted in the context of patient progression. Anti-PSMA capture leads to high capture rates of cells that remain functionally responsive to chemotherapies. These results point to the potential for microflu- idicallycaptured CTCs to inform clinical care in this patient population.