Blood transcriptional fingerprints to assess the immune status of human subjects Chapter uri icon

Overview

MeSH Major

  • Biomarkers
  • Gene Expression Profiling
  • Immune System

abstract

  • © Springer Science+Business Media, LLC 2011. All rights reserved. The blood transcriptome affords a comprehensive view of the status of the human immune system. Global changes in transcript abundance have been measured in the blood of patients with a wide range of diseases. This chapter presents an overview of the advances that have led to the identification of therapeutic targets and biomarker signatures in the field of autoimmunity and infectious disease. It also provides technology and data analysis primers as means of introducing blood transcriptome research to a broad readership. Specifically, we compare microarrays with some of the most recent digital gene expression profiling technologies available to date, including RNA sequencing. Furthermore, in addition to the basic steps involved in the analysis of microarray data we also present more advanced data mining approaches for blood transcriptional fingerprinting.

publication date

  • January 2011

Research

keywords

  • Book Chapter

Identity

Digital Object Identifier (DOI)

  • 10.1007/978-1-4419-7219-4_8

Additional Document Info

start page

  • 105

end page

  • 125