EGF shifts human airway basal cell fate toward a smoking-associated airway epithelial phenotype. Academic Article uri icon

Overview

MeSH

  • Blotting, Western
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • Microarray Analysis
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction

MeSH Major

  • Cell Differentiation
  • Epidermal Growth Factor
  • Epithelial Cells
  • Epithelial-Mesenchymal Transition
  • Receptor, Epidermal Growth Factor
  • Respiratory Mucosa
  • Smoking

abstract

  • The airway epithelium of smokers acquires pathological phenotypes, including basal cell (BC) and/or goblet cell hyperplasia, squamous metaplasia, structural and functional abnormalities of ciliated cells, decreased number of secretoglobin (SCGB1A1)-expressing secretory cells, and a disordered junctional barrier. In this study, we hypothesized that smoking alters airway epithelial structure through modification of BC function via an EGF receptor (EGFR)-mediated mechanism. Analysis of the airway epithelium revealed that EGFR is enriched in airway BCs, whereas its ligand EGF is induced by smoking in ciliated cells. Exposure of BCs to EGF shifted the BC differentiation program toward the squamous and epithelial-mesenchymal transition-like phenotypes with down-regulation of genes related to ciliogenesis, secretory differentiation, and markedly reduced junctional barrier integrity, mimicking the abnormalities present in the airways of smokers in vivo. These data suggest that activation of EGFR in airway BCs by smoking-induced EGF represents a unique mechanism whereby smoking can alter airway epithelial differentiation and barrier function.

publication date

  • July 16, 2013

has subject area

  • Blotting, Western
  • Cell Differentiation
  • Epidermal Growth Factor
  • Epithelial Cells
  • Epithelial-Mesenchymal Transition
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • Microarray Analysis
  • Real-Time Polymerase Chain Reaction
  • Receptor, Epidermal Growth Factor
  • Respiratory Mucosa
  • Reverse Transcriptase Polymerase Chain Reaction
  • Smoking

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3718120

Digital Object Identifier (DOI)

  • 10.1073/pnas.1303058110

PubMed ID

  • 23818594

Additional Document Info

start page

  • 12102

end page

  • 12107

volume

  • 110

number

  • 29