Quantification of cell cycle-arresting proteins Academic Article uri icon

Overview

MeSH Major

  • Cell Cycle Checkpoints
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Immunoblotting

abstract

  • Cellular senescence, which can be defined as a stress response preventing the propagation of cells that have accumulated potentially oncogenic alterations, is invariably associated with a permanent cell cycle arrest. Such an irreversible blockage is mainly mediated by the persistent upregulation of one or more cyclin-dependent kinase inhibitors (CKIs), including (though not limited to) p16( INK4A ) and p21( CIP1 ) and p27( KIP1 ). CKIs operate by binding to cyclin-dependent kinases (CDKs), de facto inhibiting their enzymatic activity. Here, we provide an immunoblotting-based method for the detection and quantification of CKIs in vitro and ex vivo, together with a set of guidelines for the interpretation of results.

publication date

  • January 2013

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-239-1_7

PubMed ID

  • 23296654

Additional Document Info

start page

  • 121

end page

  • 42

volume

  • 965