Gene expression analysis uncovers novel hedgehog interacting protein (HHIP) effects in human bronchial epithelial cells. Academic Article uri icon

Overview

MeSH

  • Bronchi
  • Case-Control Studies
  • Cell Line
  • Cell Proliferation
  • Extracellular Matrix Proteins
  • Gene Knockdown Techniques
  • Gene Regulatory Networks
  • Humans
  • Lung
  • Molecular Sequence Annotation
  • Oligonucleotide Array Sequence Analysis
  • RNA, Small Interfering
  • Respiratory Mucosa
  • Signal Transduction
  • Up-Regulation

MeSH Major

  • Carrier Proteins
  • Epithelial Cells
  • Membrane Glycoproteins
  • Pulmonary Disease, Chronic Obstructive
  • Transcriptome

abstract

  • Hedgehog interacting protein (HHIP) was implicated in chronic obstructive pulmonary disease (COPD) by genome-wide association studies (GWAS). However, it remains unclear how HHIP contributes to COPD pathogenesis. To identify genes regulated by HHIP, we performed gene expression microarray analysis in a human bronchial epithelial cell line (Beas-2B) stably infected with HHIP shRNAs. HHIP silencing led to differential expression of 296 genes; enrichment for variants nominally associated with COPD was found. Eighteen of the differentially expressed genes were validated by real-time PCR in Beas-2B cells. Seven of 11 validated genes tested in human COPD and control lung tissues demonstrated significant gene expression differences. Functional annotation indicated enrichment for extracellular matrix and cell growth genes. Network modeling demonstrated that the extracellular matrix and cell proliferation genes influenced by HHIP tended to be interconnected. Thus, we identified potential HHIP targets in human bronchial epithelial cells that may contribute to COPD pathogenesis. Copyright © 2013 Elsevier Inc. All rights reserved.

publication date

  • May 2013

has subject area

  • Bronchi
  • Carrier Proteins
  • Case-Control Studies
  • Cell Line
  • Cell Proliferation
  • Epithelial Cells
  • Extracellular Matrix Proteins
  • Gene Knockdown Techniques
  • Gene Regulatory Networks
  • Humans
  • Lung
  • Membrane Glycoproteins
  • Molecular Sequence Annotation
  • Oligonucleotide Array Sequence Analysis
  • Pulmonary Disease, Chronic Obstructive
  • RNA, Small Interfering
  • Respiratory Mucosa
  • Signal Transduction
  • Transcriptome
  • Up-Regulation

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3659826

Digital Object Identifier (DOI)

  • 10.1016/j.ygeno.2013.02.010

PubMed ID

  • 23459001

Additional Document Info

start page

  • 263

end page

  • 272

volume

  • 101

number

  • 5