Quantitative assessment of T cell repertoire recovery after hematopoietic stem cell transplantation Academic Article uri icon

Overview

MeSH Major

  • CD4-Positive T-Lymphocytes
  • CD8-Positive T-Lymphocytes
  • Hematopoietic Stem Cell Transplantation
  • Receptors, Antigen, T-Cell

abstract

  • Delayed T cell recovery and restricted T cell receptor (TCR) diversity after allogeneic hematopoietic stem cell transplantation (allo-HSCT) are associated with increased risks of infection and cancer relapse. Technical challenges have limited faithful measurement of TCR diversity after allo-HSCT. Here we combined 5' rapid amplification of complementary DNA ends PCR with deep sequencing to quantify TCR diversity in 28 recipients of allo-HSCT using a single oligonucleotide pair. Analysis of duplicate blood samples confirmed that we accurately determined the frequency of individual TCRs. After 6 months, cord blood-graft recipients approximated the TCR diversity of healthy individuals, whereas recipients of T cell-depleted peripheral-blood stem cell grafts had 28-fold and 14-fold lower CD4(+) and CD8(+) T cell diversities, respectively. After 12 months, these deficiencies had improved for the CD4(+) but not the CD8(+) T cell compartment. Overall, this method provides unprecedented views of T cell repertoire recovery after allo-HSCT and may identify patients at high risk of infection or relapse.

publication date

  • March 2013

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3594333

Digital Object Identifier (DOI)

  • 10.1038/nm.3100

PubMed ID

  • 23435170

Additional Document Info

start page

  • 372

end page

  • 7

volume

  • 19

number

  • 3