Transgenerational cell fate profiling: A method for the graphical presentation of complex cell cycle alterations Academic Article uri icon

Overview

MeSH Major

  • Anthracenes
  • Cell Cycle Checkpoints
  • Protein Kinase Inhibitors

abstract

  • The illicit generation of tetraploid cells constitutes a prominent driver of oncogenesis, as it often precedes the development of aneuploidy and genomic instability. In addition, tetraploid (pre-)malignant cells display an elevated resistance against radio- and chemotherapy. Here, we report a strategy to preferentially kill tetraploid tumor cells based on the broad-spectrum kinase inhibitor SP600125. Live videomicroscopy revealed that SP600125 affects the execution of mitosis, impedes proper cell division and/or activates apoptosis in near-to-tetraploid, though less so in parental, cancer cells. We propose a novel graphical model to quantify the differential response of diploid and tetraploid cells to mitotic perturbators, including SP600125, which we baptized "transgenerational cell fate profiling." We speculate that this representation constitutes a valid alternative to classical "single-cell fate" and "genealogical" profiling and, hence, may facilitate the analysis of cell fate within a heterogeneous population as well as the visual examination of cell cycle alterations.

publication date

  • January 2013

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3570510

Digital Object Identifier (DOI)

  • 10.4161/cc.23046

PubMed ID

  • 23255111

Additional Document Info

start page

  • 183

end page

  • 90

volume

  • 12

number

  • 1