Regulation of pluripotency and cellular reprogramming by the ubiquitin-proteasome system Academic Article Article uri icon

Overview

MeSH Major

  • Carcinoma, Papillary
  • Thyroid Neoplasms

abstract

  • Although transcriptional regulation of stem cell pluripotency and differentiation has been extensively studied, only a small number of studies have addressed the roles for posttranslational modifications in these processes. A key mechanism of posttranslational modification is ubiquitination by the ubiquitin-proteasome system (UPS). Here, using shotgun proteomics, we map the ubiquitinated protein landscape during embryonic stem cell (ESC) differentiation and induced pluripotency. Moreover, using UPS-targeted RNAi screens, we identify additional regulators of pluripotency and differentiation. We focus on two of these proteins, the deubiquitinating enzyme Psmd14 and the E3 ligase Fbxw7, and characterize their importance in ESC pluripotency and cellular reprogramming. This global characterization of the UPS as a key regulator of stem cell pluripotency opens the way for future studies that focus on specific UPS enzymes or ubiquitinated substrates. © 2012 Elsevier Inc.

publication date

  • December 7, 2012

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/j.stem.2012.09.011

PubMed ID

  • 23103054

Additional Document Info

start page

  • 783

end page

  • 798

volume

  • 11

number

  • 6