Genome-wide identification of miRNA targets by PAR-CLIP Academic Article uri icon

Overview

MeSH Major

  • Argonaute Proteins
  • MicroRNAs
  • RNA, Messenger
  • Ribonucleosides

abstract

  • miRNAs are short (20-23 nt) RNAs that are loaded into proteins of the Argonaute (AGO) family and guide them to partially complementary target sites on mRNAs, resulting in mRNA destabilization and/or translational repression. It is estimated that about 60% of the mammalian genes are potentially regulated by miRNAs, and therefore methods for experimental miRNA target determination have become valuable tools for the characterization of posttranscriptional gene regulation. Here we present a step-by-step protocol and guidelines for the computational analysis for the large-scale identification of miRNA target sites in cultured cells by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP) of AGO proteins.

publication date

  • October 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3508682

Digital Object Identifier (DOI)

  • 10.1016/j.ymeth.2012.08.006

PubMed ID

  • 22926237

Additional Document Info

start page

  • 94

end page

  • 105

volume

  • 58

number

  • 2