Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells Academic Article uri icon

Overview

MeSH Major

  • Cell Separation
  • Microfluidic Analytical Techniques
  • Neoplastic Stem Cells
  • Spheroids, Cellular

abstract

  • Here we report a microfluidics method to enrich physically deformable cells by mechanical manipulation through artificial microbarriers. Driven by hydrodynamic forces, flexible cells or cells with high metastatic propensity change shape to pass through the microbarriers and exit the separation device, whereas stiff cells remain trapped. We demonstrate the separation of (i) a mixture of two breast cancer cell types (MDA-MB-436 and MCF-7) with distinct deformabilities and metastatic potentials, and (ii) a heterogeneous breast cancer cell line (SUM149), into enriched flexible and stiff subpopulations. We show that the flexible phenotype is associated with overexpression of multiple genes involved in cancer cell motility and metastasis, and greater mammosphere formation efficiency. Our observations support the relationship between tumor-initiating capacity and cell deformability, and demonstrate that tumor-initiating cells are less differentiated in terms of cell biomechanics.

publication date

  • November 13, 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3503214

Digital Object Identifier (DOI)

  • 10.1073/pnas.1209893109

PubMed ID

  • 23112172

Additional Document Info

start page

  • 18707

end page

  • 12

volume

  • 109

number

  • 46