Mass spectrometry tools for analysis of intermolecular interactions Academic Article uri icon


MeSH Major

  • Mass Spectrometry
  • Proteins


  • The small quantities of protein required for mass spectrometry (MS) make it a powerful tool to detect binding (protein-protein, protein-small molecule, etc.) of proteins that are difficult to express in large quantities, as is the case for many intrinsically disordered proteins. Chemical cross-linking, proteolysis, and MS analysis, combined, are a powerful tool for the identification of binding domains. Here, we present a traditional approach to determine protein-protein interaction binding sites using heavy water ((18)O) as a label. This technique is relatively inexpensive and can be performed on any mass spectrometer without specialized software.

publication date

  • August 29, 2012



  • Academic Article



  • eng

PubMed Central ID

  • PMC4638115

Digital Object Identifier (DOI)

  • 10.1007/978-1-4614-3704-8_26

PubMed ID

  • 22821539

Additional Document Info

start page

  • 387

end page

  • 98


  • 896