Development of a vascular niche platform for expansion of repopulating human cord blood stem and progenitor cells. Academic Article Article uri icon

Overview

MeSH

  • Animals
  • Cell Culture Techniques
  • Cells, Cultured
  • Cord Blood Stem Cell Transplantation
  • Humans
  • Mice
  • Mice, Inbred NOD
  • Mice, Transgenic
  • Tissue Engineering

MeSH Major

  • Blood Vessels
  • Cell Proliferation
  • Fetal Blood
  • Hematopoietic Stem Cells
  • Stem Cell Niche
  • Tissue Scaffolds

abstract

  • Transplantation of ex vivo expanded human umbilical cord blood cells (hCB) only partially enhances the hematopoietic recovery after myelosuppressive therapy. Incubation of hCB with optimal combinations of cytokines and niche cells, such as endothelial cells (ECs), could augment the efficiency of hCB expansion. We have devised an approach to cultivate primary human ECs (hECs) in serum-free culture conditions. We demonstrate that coculture of CD34(+) hCB in direct cellular contact with hECs and minimal concentrations of thrombopoietin/Kit-ligand/Flt3-ligand resulted in a 400-fold expansion of total hematopoietic cells, 150-fold expansion of CD45(+)CD34(+) progenitor cells, and 23-fold expansion of CD45(+) Lin(-)CD34(hi+)CD45RA(-)CD49f(+) stem and progenitor cells over a 12-day period. Compared with cytokines alone, coculture of hCB with hECs permitted greater expansion of cells capable of multilineage engraftment and serial transplantation, hallmarks of long-term repopulating hematopoietic stem cells. Therefore, hECs establish a cellular platform for expansion of hematopoietic stem and progenitor cells and treatment of hematologic disorders.

publication date

  • August 9, 2012

has subject area

  • Animals
  • Blood Vessels
  • Cell Culture Techniques
  • Cell Proliferation
  • Cells, Cultured
  • Cord Blood Stem Cell Transplantation
  • Fetal Blood
  • Hematopoietic Stem Cells
  • Humans
  • Mice
  • Mice, Inbred NOD
  • Mice, Transgenic
  • Stem Cell Niche
  • Tissue Engineering
  • Tissue Scaffolds

Research

keywords

  • Evaluation Studies
  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3418723

Digital Object Identifier (DOI)

  • 10.1182/blood-2011-12-398115

PubMed ID

  • 22709690

Additional Document Info

start page

  • 1344

end page

  • 1347

volume

  • 120

number

  • 6