A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans Academic Article uri icon

Overview

MeSH Major

  • Anion Transport Proteins
  • Drosophila Proteins
  • Drosophila melanogaster
  • Mitochondria
  • Mitochondrial Membrane Transport Proteins
  • Mitochondrial Membranes
  • Mitochondrial Proteins
  • Pyruvic Acid
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins

abstract

  • Pyruvate constitutes a critical branch point in cellular carbon metabolism. We have identified two proteins, Mpc1 and Mpc2, as essential for mitochondrial pyruvate transport in yeast, Drosophila, and humans. Mpc1 and Mpc2 associate to form an ~150-kilodalton complex in the inner mitochondrial membrane. Yeast and Drosophila mutants lacking MPC1 display impaired pyruvate metabolism, with an accumulation of upstream metabolites and a depletion of tricarboxylic acid cycle intermediates. Loss of yeast Mpc1 results in defective mitochondrial pyruvate uptake, and silencing of MPC1 or MPC2 in mammalian cells impairs pyruvate oxidation. A point mutation in MPC1 provides resistance to a known inhibitor of the mitochondrial pyruvate carrier. Human genetic studies of three families with children suffering from lactic acidosis and hyperpyruvatemia revealed a causal locus that mapped to MPC1, changing single amino acids that are conserved throughout eukaryotes. These data demonstrate that Mpc1 and Mpc2 form an essential part of the mitochondrial pyruvate carrier.

publication date

  • July 6, 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3690818

Digital Object Identifier (DOI)

  • 10.1126/science.1218099

PubMed ID

  • 22628558

Additional Document Info

start page

  • 96

end page

  • 100

volume

  • 336

number

  • 6090