Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis Academic Article uri icon

Overview

MeSH Major

  • Amyloidosis
  • Kidney Diseases
  • Kidney Glomerulus
  • Laser Capture Microdissection
  • Proteins
  • Proteomics
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry

abstract

  • Accurate diagnosis and typing of renal amyloidosis is critical for prognosis, genetic counseling, and treatment. Laser microdissection and mass spectrometry are emerging techniques for the analysis and diagnosis of many renal diseases. Here we present the results of laser microdissection and mass spectrometry performed on 127 cases of renal amyloidosis during 2008-2010. We found the following proteins in the amyloid deposits: immunoglobulin light and heavy chains, secondary reactive serum amyloid A protein, leukocyte cell-derived chemotaxin-2, fibrinogen-α chain, transthyretin, apolipoprotein A-I and A-IV, gelsolin, and β-2 microglobulin. Thus, laser microdissection of affected areas within the kidney followed by mass spectrometry provides a direct test of the composition of the deposit and forms a useful ancillary technique for the accurate diagnosis and typing of renal amyloidosis in a single procedure.

publication date

  • July 2, 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3388518

Digital Object Identifier (DOI)

  • 10.1038/ki.2012.108

PubMed ID

  • 22495291

Additional Document Info

start page

  • 226

end page

  • 34

volume

  • 82

number

  • 2