Bioorthogonal imaging of Aurora Kinase A in live cells Academic Article uri icon

Overview

MeSH Major

  • Molecular Imaging
  • Protein-Serine-Threonine Kinases

abstract

  • In living color: Aurora kinase A (AKA) was imaged in live cells using a bioorthogonal two-step reaction with a small molecule AKA inhibitor (see scheme) and a fluorescent reporter. The fluorescent molecule was localized to spindle poles and microtubules during metaphase, consistent with the localization of both endogenous and green fluorescent protein tagged AKA. By using this approach, changes in AKA distribution during mitosis were also observed. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

publication date

  • July 2, 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3523717

Digital Object Identifier (DOI)

  • 10.1002/anie.201200994

PubMed ID

  • 22644700

Additional Document Info

start page

  • 6598

end page

  • 603

volume

  • 51

number

  • 27