Bioorthogonal imaging of Aurora Kinase A in live cells
In living color: Aurora kinase A (AKA) was imaged in live cells using a bioorthogonal two-step reaction with a small molecule AKA inhibitor (see scheme) and a fluorescent reporter. The fluorescent molecule was localized to spindle poles and microtubules during metaphase, consistent with the localization of both endogenous and green fluorescent protein tagged AKA. By using this approach, changes in AKA distribution during mitosis were also observed. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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