Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates. Academic Article uri icon

Overview

MeSH

  • Cell Line, Tumor
  • Equipment Design
  • Humans
  • Spheroids, Cellular

MeSH Major

  • Cell Culture Techniques
  • High-Throughput Screening Assays

abstract

  • Using stereolithography, 20 different structural variations comprised of millimeter diameter holes surrounded by trenches, plateaus, or micro-ring structures were prepared and tested for their ability to stably hold arrays of microliter sized droplets within the structures over an extended period of time. The micro-ring structures were the most effective in stabilizing droplets against mechanical and chemical perturbations. After confirming the importance of micro-ring structures using rapid prototyping, we developed an injection molding tool for mass production of polystyrene 3D cell culture plates with an array of 384 such micro-ring surrounded through-hole structures. These newly designed and injection molded polystyrene 384 hanging drop array plates with micro-rings were stable and robust against mechanical perturbations as well as surface fouling-facilitated droplet spreading making them capable of long term cell spheroid culture of up to 22┬ádays within the droplet array. This is a significant improvement over previously reported 384 hanging drop array plates which are susceptible to small mechanical shocks and could not reliably maintain hanging drops for longer than a few days. With enhanced droplet stability, the hanging drop array plates with micro-ring structures provide better platforms and open up new opportunities for high-throughput preparation of microscale 3D cell constructs for drug screening and cell analysis.

publication date

  • April 2012

has subject area

  • Cell Culture Techniques
  • Cell Line, Tumor
  • Equipment Design
  • High-Throughput Screening Assays
  • Humans
  • Spheroids, Cellular

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3304008

Digital Object Identifier (DOI)

  • 10.1007/s10544-011-9608-5

PubMed ID

  • 22057945

Additional Document Info

start page

  • 313

end page

  • 323

volume

  • 14

number

  • 2