Imaging therapeutic PARP inhibition in vivo through bioorthogonally developed companion imaging agents Academic Article Article uri icon


MeSH Major

  • Antineoplastic Agents
  • Attitude to Health
  • Drug-Related Side Effects and Adverse Reactions
  • Neoplasm Recurrence, Local
  • Ovarian Neoplasms
  • Patient Care Planning
  • Quality of Life
  • Survivors


  • A number of small-molecule poly (ADP-ribose) polymerase (PARP) inhibitors are currently undergoing advanced clinical trials. Determining the distribution and target inhibitory activity of these drugs in individual subjects, however, has proven problematic. Here, we used a PARP agent for positron emission tomography-computed tomography (PET-CT) imaging ((18)F-BO), which we developed based on the Olaparib scaffold using rapid bioorthogonal conjugation chemistries. We show that the bioorthogonal (18)F modification of the parent molecule is simple, highly efficient, and well tolerated, resulting in a half maximal inhibitory concentration (IC(50)) of 17.9 ± 1.1 nM. Intravital imaging showed ubiquitous distribution of the drug and uptake into cancer cells, with ultimate localization within the nucleus, all of which were inhibitable. Whole-body PET-CT imaging showed tumoral uptake of the drug, which decreased significantly, after a daily dose of Olaparib. Standard (18)F-fludeoxyglucose imaging, however, failed to detect such therapy-induced changes. This research represents a step toward developing a more generic approach for the rapid codevelopment of companion imaging agents based on small-molecule therapeutic inhibitors.

publication date

  • March 2012



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1593/neo.12414

PubMed ID

  • 22496617

Additional Document Info

start page

  • 169

end page

  • 77


  • 14


  • 3