BDNF Val66Met impairs fluoxetine-induced enhancement of adult hippocampus plasticity. Academic Article uri icon

Overview

MeSH

  • Analysis of Variance
  • Animals
  • Biophysics
  • Bromodeoxyuridine
  • Electric Stimulation
  • Enzyme-Linked Immunosorbent Assay
  • Fluoxetine
  • Gene Expression Regulation
  • Glial Fibrillary Acidic Protein
  • Humans
  • In Vitro Techniques
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Patch-Clamp Techniques
  • Receptor, trkB
  • Serotonin Uptake Inhibitors

MeSH Major

  • Brain-Derived Neurotrophic Factor
  • Dentate Gyrus
  • Long-Term Potentiation
  • Methionine
  • Polymorphism, Single Nucleotide
  • Valine

abstract

  • Recently, a single-nucleotide polymorphism (SNP) in the brain-derived neurotrophic factor (BDNF) gene (BDNF Val66Met) has been linked to the development of multiple forms of neuropsychiatric illness. This SNP, when genetically introduced into mice, recapitulates core phenotypes identified in human BDNF Val66Met carriers. In mice, this SNP also leads to elevated expression of anxiety-like behaviors that are not rescued with the prototypic selective serotonin reuptake inhibitor (SSRI), fluoxetine. A prominent hypothesis is that SSRI-induced augmentation of BDNF protein expression and the beneficial trophic effects of BDNF on neural plasticity are critical components for drug response. Thus, these mice represent a potential model to study the biological mechanism underlying treatment-resistant forms of affective disorders. To test whether the BDNF Val66Met SNP alters SSRI-induced changes in neural plasticity, we used wild-type (BDNF(Val/Val)) mice, and mice homozygous for the BDNF Val66Met SNP (BDNF(Met/Met)). We assessed hippocampal BDNF protein levels, survival rates of adult born cells, and synaptic plasticity (long-term potentiation, LTP) in the dentate gyrus either with or without chronic (28-day) fluoxetine treatment. BDNF(Met/Met) mice had decreased basal BDNF protein levels in the hippocampus that did not significantly increase following fluoxetine treatment. BDNF(Met/Met) mice had impaired survival of newly born cells and LTP in the dentate gyrus; the LTP effects remained blunted following fluoxetine treatment. The observed effects of the BDNF Val66Met SNP on hippocampal BDNF expression and synaptic plasticity provide a possible mechanistic basis by which this common BDNF SNP may impair efficacy of SSRI drug treatment.

publication date

  • April 2012

has subject area

  • Analysis of Variance
  • Animals
  • Biophysics
  • Brain-Derived Neurotrophic Factor
  • Bromodeoxyuridine
  • Dentate Gyrus
  • Electric Stimulation
  • Enzyme-Linked Immunosorbent Assay
  • Fluoxetine
  • Gene Expression Regulation
  • Glial Fibrillary Acidic Protein
  • Humans
  • In Vitro Techniques
  • Long-Term Potentiation
  • Male
  • Methionine
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Patch-Clamp Techniques
  • Polymorphism, Single Nucleotide
  • Receptor, trkB
  • Serotonin Uptake Inhibitors
  • Valine

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3306891

Digital Object Identifier (DOI)

  • 10.1038/npp.2011.318

PubMed ID

  • 22218094

Additional Document Info

start page

  • 1297

end page

  • 1304

volume

  • 37

number

  • 5