Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells Academic Article uri icon

Overview

MeSH Major

  • Adipocytes
  • DNA-Binding Proteins
  • Endothelial Cells
  • Pericytes
  • Stem Cells
  • Transcription Factors

abstract

  • Progress has been made in elucidating the cell-surface phenotype of primary adipose progenitors; however, specific functional markers and distinct molecular signatures of fat depot-specific preadipocytes have remained elusive. In this study, we label committed murine adipose progenitors through expression of GFP from the genetic locus for Zfp423, a gene controlling preadipocyte determination. Selection of GFP-expressing fibroblasts from either subcutaneous or visceral adipose-derived stromal vascular cultures isolates stably committed preadipocytes that undergo robust adipogenesis. Immunohistochemistry for Zfp423-driven GFP expression in┬ávivo confirms a perivascular origin of preadipocytes within both white and brown adipose tissues. Interestingly, a small subset of capillary endothelial cells within white and brown fat also express this marker, suggesting a contribution of specialized endothelial cells to the adipose lineage. Zfp423(GFP) mice represent a simple tool for the specific localization and isolation of molecularly defined preadipocytes from distinct adipose tissue depots.

publication date

  • February 8, 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3366493

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2012.01.010

PubMed ID

  • 22326224

Additional Document Info

start page

  • 230

end page

  • 9

volume

  • 15

number

  • 2