Cyanine fluorophore derivatives with enhanced photostability Academic Article uri icon

Overview

MeSH Major

  • Carbocyanines
  • Fluorescent Dyes

abstract

  • Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.

publication date

  • January 2012

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3433158

Digital Object Identifier (DOI)

  • 10.1038/nmeth.1774

PubMed ID

  • 22081126

Additional Document Info

start page

  • 68

end page

  • 71

volume

  • 9

number

  • 1