Cyanine fluorophore derivatives with enhanced photostability. Academic Article uri icon

Overview

MeSH

  • Animals
  • Benzyl Alcohols
  • CHO Cells
  • Chromans
  • Cricetinae
  • Cyclooctanes
  • DNA
  • Drug Stability
  • Fluorescence
  • Humans
  • Kinetics
  • Molecular Dynamics Simulation
  • Photobleaching
  • Receptors, Dopamine D2
  • Singlet Oxygen

MeSH Major

  • Carbocyanines
  • Fluorescent Dyes

abstract

  • Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.

publication date

  • November 13, 2011

has subject area

  • Animals
  • Benzyl Alcohols
  • CHO Cells
  • Carbocyanines
  • Chromans
  • Cricetinae
  • Cyclooctanes
  • DNA
  • Drug Stability
  • Fluorescence
  • Fluorescent Dyes
  • Humans
  • Kinetics
  • Molecular Dynamics Simulation
  • Photobleaching
  • Receptors, Dopamine D2
  • Singlet Oxygen

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3433158

Digital Object Identifier (DOI)

  • 10.1038/nmeth.1774

PubMed ID

  • 22081126

Additional Document Info

start page

  • 68

end page

  • 71

volume

  • 9

number

  • 1