Bioorthogonal probes for polo-like kinase 1 imaging and quantification
Cell Cycle Proteins
Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PubMed Central ID
Digital Object Identifier (DOI)
Additional Document Info
has global citation frequency