Bioorthogonal probes for polo-like kinase 1 imaging and quantification Academic Article uri icon

Overview

MeSH Major

  • Cell Cycle Proteins
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins
  • Pteridines

abstract

  • Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

publication date

  • September 26, 2011

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3187564

Digital Object Identifier (DOI)

  • 10.1002/anie.201103273

PubMed ID

  • 21948435

Additional Document Info

start page

  • 9378

end page

  • 81

volume

  • 50

number

  • 40