Structures of the bacterial ribosome in classical and hybrid states of tRNA binding. Academic Article uri icon

Overview

MeSH

  • Anticodon
  • Crystallography, X-Ray
  • Escherichia coli
  • Escherichia coli Proteins
  • Models, Molecular
  • Nucleic Acid Conformation
  • Protein Biosynthesis
  • RNA, Messenger
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S
  • RNA, Transfer, Amino Acyl
  • Ribosomal Proteins

MeSH Major

  • RNA, Bacterial
  • RNA, Transfer, Phe
  • Ribosome Subunits, Large, Bacterial
  • Ribosome Subunits, Small, Bacterial

abstract

  • During protein synthesis, the ribosome controls the movement of tRNA and mRNA by means of large-scale structural rearrangements. We describe structures of the intact bacterial ribosome from Escherichia coli that reveal how the ribosome binds tRNA in two functionally distinct states, determined to a resolution of ~3.2 angstroms by means of x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit site. The structures help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and ribosome recycling.

publication date

  • May 20, 2011

has subject area

  • Anticodon
  • Crystallography, X-Ray
  • Escherichia coli
  • Escherichia coli Proteins
  • Models, Molecular
  • Nucleic Acid Conformation
  • Protein Biosynthesis
  • RNA, Bacterial
  • RNA, Messenger
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S
  • RNA, Transfer, Amino Acyl
  • RNA, Transfer, Phe
  • Ribosomal Proteins
  • Ribosome Subunits, Large, Bacterial
  • Ribosome Subunits, Small, Bacterial

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3176341

Digital Object Identifier (DOI)

  • 10.1126/science.1202692

PubMed ID

  • 21596992

Additional Document Info

start page

  • 981

end page

  • 984

volume

  • 332

number

  • 6032