Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation. Academic Article uri icon

Overview

MeSH

  • Cryoelectron Microscopy
  • Mutation
  • Peptide Chain Elongation, Translational

MeSH Major

  • Escherichia coli
  • RNA, Bacterial
  • RNA, Messenger
  • RNA, Transfer
  • Ribosomes

abstract

  • mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.

publication date

  • March 22, 2011

has subject area

  • Cryoelectron Microscopy
  • Escherichia coli
  • Mutation
  • Peptide Chain Elongation, Translational
  • RNA, Bacterial
  • RNA, Messenger
  • RNA, Transfer
  • Ribosomes

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3064370

Digital Object Identifier (DOI)

  • 10.1073/pnas.1101503108

PubMed ID

  • 21383139

Additional Document Info

start page

  • 4817

end page

  • 4821

volume

  • 108

number

  • 12