Transcriptional and posttranslational up-regulation of HER3 (ErbB3) compensates for inhibition of the HER2 tyrosine kinase Academic Article uri icon

Overview

MeSH Major

  • Breast Neoplasms
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Neoplastic
  • Protein Kinase Inhibitors
  • Quinazolines
  • Receptor, ErbB-2
  • Receptor, ErbB-3
  • Transcription, Genetic
  • Up-Regulation

abstract

  • Sustained and complete inhibition of HER3 and its output to PI3K/Akt are required for the optimal antitumor effect of therapeutic inhibitors of the HER2 oncogene. Here, we show that, after inhibition of the HER2 tyrosine kinase with lapatinib, there is PI3K/Akt and FoxO3a-dependent up-regulation of HER3 mRNA and protein. Up-regulated HER3 was then phosphorylated by residual HER2 activity, thus partially maintaining P-Akt and limiting the antitumor action of lapatinib. Inhibition of HER3 with siRNA or a neutralizing HER3 antibody sensitized HER2+ breast cancer cells and xenografts to lapatinib both in vitro and in vivo. Combined blockade of HER2 and HER3 inhibited pharmacodynamic biomarkers of PI3K/Akt activity more effectively than each inhibitor alone. These results suggest that because of HER3-mediated compensation, current clinical inhibitors of HER2 and PI3K/Akt will not block the PI3K pathway completely. They also suggest that therapeutic inhibitors of HER3 should be used in combination with HER2 inhibitors and PI3K pathway inhibitors in patients with HER2- and PI3K-dependent cancers.

publication date

  • March 22, 2011

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3064360

Digital Object Identifier (DOI)

  • 10.1073/pnas.1016140108

PubMed ID

  • 21385943

Additional Document Info

start page

  • 5021

end page

  • 6

volume

  • 108

number

  • 12