Resistance of a human immunodeficiency virus type 1 isolate to a small molecule CCR5 inhibitor can involve sequence changes in both gp120 and gp41 Academic Article uri icon

Overview

MeSH Major

  • Amino Acid Substitution
  • CCR5 Receptor Antagonists
  • Drug Resistance, Viral
  • HIV Envelope Protein gp120
  • HIV Envelope Protein gp41
  • HIV-1
  • Piperazines
  • Pyrimidines

abstract

  • Here, we describe the genetic pathways taken by a human immunodeficiency virus type 1 (HIV-1) isolate, D101.12, to become resistant to the small molecule CCR5 inhibitor, vicriviroc (VCV), in vitro. Resistant D101.12 variants contained at least one substitution in the gp120 V3 region (H308P), plus one of two patterns of gp41 sequence changes involving the fusion peptide (FP) and a downstream residue: G514V+V535M or M518V+F519L+V535M. Studies of Env-chimeric and point-substituted viruses in peripheral blood mononuclear cells (PBMC) and TZM-bl cells showed that resistance can arise from the cooperative action of gp120 and gp41 changes, while retaining CCR5 usage. Modeling the VCV inhibition data from the two cell types suggests that D101.12 discriminates between high- and low-VCV affinity forms of CCR5 less than D1/85.16, a resistant virus with three FP substitutions.

publication date

  • April 25, 2011

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3070043

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2010.12.052

PubMed ID

  • 21356539

Additional Document Info

start page

  • 47

end page

  • 59

volume

  • 413

number

  • 1