Effect of brain-derived neurotrophic factor haploinsufficiency on stress-induced remodeling of hippocampal neurons. Academic Article uri icon

Overview

MeSH

  • Animals
  • CA1 Region, Hippocampal
  • Corticosterone
  • Dendritic Spines
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Animal
  • Neurons
  • Organ Size
  • Rats

MeSH Major

  • Brain-Derived Neurotrophic Factor
  • CA3 Region, Hippocampal
  • Haploinsufficiency
  • Pyramidal Cells
  • Stress, Psychological

abstract

  • Chronic restraint stress (CRS) induces the remodeling (i.e., retraction and simplification) of the apical dendrites of hippocampal CA3 pyramidal neurons in rats, suggesting that intrahippocampal connectivity can be affected by a prolonged stressful challenge. Since the structural maintenance of neuronal dendritic arborizations and synaptic connectivity requires neurotrophic support, we investigated the potential role of brain derived neurotrophic factor (BDNF), a neurotrophin enriched in the hippocampus and released from neurons in an activity-dependent manner, as a mediator of the stress-induced dendritic remodeling. The analysis of Golgi-impregnated hippocampal sections revealed that wild type (WT) C57BL/6 male mice showed a similar CA3 apical dendritic remodeling in response to three weeks of CRS to that previously described for rats. Haploinsufficient BDNF mice (BDNF(±) ) did not show such remodeling, but, even without CRS, they presented shorter and simplified CA3 apical dendritic arbors, like those observed in stressed WT mice. Furthermore, unstressed BDNF(±) mice showed a significant decrease in total hippocampal volume. The dendritic arborization of CA1 pyramidal neurons was not affected by CRS or genotype. However, only in WT mice, CRS induced changes in the density of dendritic spine shape subtypes in both CA1 and CA3 apical dendrites. These results suggest a complex role of BDNF in maintaining the dendritic and spine morphology of hippocampal neurons and the associated volume of the hippocampal formation. The inability of CRS to modify the dendritic structure of CA3 pyramidal neurons in BDNF(±) mice suggests an indirect, perhaps permissive, role of BDNF in mediating hippocampal dendritic remodeling. Copyright © 2010 Wiley-Liss, Inc.

publication date

  • March 2011

has subject area

  • Animals
  • Brain-Derived Neurotrophic Factor
  • CA1 Region, Hippocampal
  • CA3 Region, Hippocampal
  • Corticosterone
  • Dendritic Spines
  • Haploinsufficiency
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Animal
  • Neurons
  • Organ Size
  • Pyramidal Cells
  • Rats
  • Stress, Psychological

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2888762

Digital Object Identifier (DOI)

  • 10.1002/hipo.20744

PubMed ID

  • 20095008

Additional Document Info

start page

  • 253

end page

  • 264

volume

  • 21

number

  • 3