MicroRNA-335 inhibits tumor reinitiation and is silenced through genetic and epigenetic mechanisms in human breast cancer Academic Article uri icon


MeSH Major

  • Breast Neoplasms
  • Epigenesis, Genetic
  • MicroRNAs
  • Neoplasm Metastasis
  • RNA Interference


  • Post-transcriptional regulators have emerged as robust effectors of metastasis and display deregulated expression through unknown mechanisms. Here, we reveal that the human microRNA-335 locus undergoes genetic deletion and epigenetic promoter hypermethylation in every metastatic derivative obtained from independent patients' malignant cell populations. Genetic deletion of miR-335 is a common event in human breast cancer, is enriched for in breast cancer metastases, and also correlates with ovarian cancer recurrence. We furthermore identify miR-335 as a robust inhibitor of tumor reinitiation. We thus implicate the miR-335 locus on 7q32.2 as the first selective metastasis suppressor and tumor initiation suppressor locus in human breast cancer.

publication date

  • February 2011



  • Academic Article



  • eng

PubMed Central ID

  • PMC3034897

Digital Object Identifier (DOI)

  • 10.1101/gad.1974211

PubMed ID

  • 21289068

Additional Document Info

start page

  • 226

end page

  • 31


  • 25


  • 3