The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia. Academic Article uri icon

Overview

MeSH

  • Bronchi
  • Cell Culture Techniques
  • Culture Media
  • Genome, Human
  • Humans
  • Tissue Donors
  • Trachea

MeSH Major

  • Epithelial Cells
  • Gene Expression Profiling
  • Transcription, Genetic

abstract

  • Organotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity, transcytosis, infection, inflammation, signaling, cilia, and repair functions of this complex tissue. However, we do not know how closely these cultures resemble the airway surface epithelium in vivo. In this study, we examined the genome-wide expression profile of tracheal and bronchial human airway epithelia in vivo and compared it with the expression profile of primary cultures of human airway epithelia grown at the air-liquid interface. For comparison, we also investigated the expression profile of Calu-3 cells grown at the air-liquid interface and primary cultures of human airway epithelia submerged in nutrient media. We found that the transcriptional profile of differentiated primary cultures grown at the air-liquid interface most closely resembles that of in vivo airway epithelia, suggesting that the use of primary cultures and the presence of an air-liquid interface are important to recapitulate airway epithelia biology. We describe a high level of similarity between cells of tracheal and bronchial origin within and between different human donors, which suggests a very robust expression profile that is specific to airway cells.

publication date

  • January 2011

has subject area

  • Bronchi
  • Cell Culture Techniques
  • Culture Media
  • Epithelial Cells
  • Gene Expression Profiling
  • Genome, Human
  • Humans
  • Tissue Donors
  • Trachea
  • Transcription, Genetic

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3023285

Digital Object Identifier (DOI)

  • 10.1152/ajplung.00256.2010

PubMed ID

  • 20971803

Additional Document Info

start page

  • L25

end page

  • L31

volume

  • 300

number

  • 1