Hydrodynamic cellular patterning for 3D co-culture
Attitude of Health Personnel
Education, Medical, Graduate
This paper describes a microfluidic cell patterning method that enables spatial control of the formation of co-cultured spheroids, which is the self-organization of cells into 3D aggregates. Multiple laminar flows are utilized to simultaneously pat-tern separate populations of cells in well defined spatial arrangements. The pat-terned spheroids are able to continuously grow in their defined locations. This en-ables long-term monitoring of the spheroids including the self-aggregation process to form co-cultured spheroids and other heterogeneous cellular interactions. This 3D cellular patterning method facilitates better understanding of interactions between multiple cell types by providing an environment similar to that inside the body. © 2008 CBMS.
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