Endophilin functions as a membrane-bending molecule and is delivered to endocytic zones by exocytosis Academic Article uri icon


MeSH Major

  • Caenorhabditis elegans
  • Endocytosis
  • Exocytosis
  • Synaptic Vesicles


  • Two models have been proposed for endophilin function in synaptic vesicle (SV) endocytosis. The scaffolding model proposes that endophilin's SH3 domain recruits essential endocytic proteins, whereas the membrane-bending model proposes that the BAR domain induces positively curved membranes. We show that mutations disrupting the scaffolding function do not impair endocytosis, whereas those disrupting membrane bending cause significant defects. By anchoring endophilin to the plasma membrane, we show that endophilin acts prior to scission to promote endocytosis. Despite acting at the plasma membrane, the majority of endophilin is targeted to the SV pool. Photoactivation studies suggest that the soluble pool of endophilin at synapses is provided by unbinding from the adjacent SV pool and that the unbinding rate is regulated by exocytosis. Thus, endophilin participates in an association-dissociation cycle with SVs that parallels the cycle of exo- and endocytosis. This endophilin cycle may provide a mechanism for functionally coupling endocytosis and exocytosis.

publication date

  • October 29, 2010



  • Academic Article



  • eng

PubMed Central ID

  • PMC2996235

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2010.09.024

PubMed ID

  • 21029864

Additional Document Info

start page

  • 430

end page

  • 41


  • 143


  • 3