Complement receptor 2/CD21- human naive B cells contain mostly autoreactive unresponsive clones Academic Article uri icon


MeSH Major

  • Autoimmune Diseases
  • B-Lymphocytes
  • Biomarkers
  • Clonal Anergy
  • Receptors, Complement 3d


  • Complement receptor 2-negative (CR2/CD21(-)) B cells have been found enriched in patients with autoimmune diseases and in common variable immunodeficiency (CVID) patients who are prone to autoimmunity. However, the physiology of CD21(-/lo) B cells remains poorly characterized. We found that some rheumatoid arthritis (RA) patients also display an increased frequency of CD21(-/lo) B cells in their blood. A majority of CD21(-/lo) B cells from RA and CVID patients expressed germline autoreactive antibodies, which recognized nuclear and cytoplasmic structures. In addition, these B cells were unable to induce calcium flux, become activated, or proliferate in response to B-cell receptor and/or CD40 triggering, suggesting that these autoreactive B cells may be anergic. Moreover, gene array analyses of CD21(-/lo) B cells revealed molecules specifically expressed in these B cells and that are likely to induce their unresponsive stage. Thus, CD21(-/lo) B cells contain mostly autoreactive unresponsive clones, which express a specific set of molecules that may represent new biomarkers to identify anergic B cells in humans.

publication date

  • June 17, 2010



  • Academic Article



  • eng

PubMed Central ID

  • PMC3373152

Digital Object Identifier (DOI)

  • 10.1182/blood-2009-09-243071

PubMed ID

  • 20231422

Additional Document Info

start page

  • 5026

end page

  • 36


  • 115


  • 24