PAR-2 receptor-induced effects on human eccrine sweat gland cells Article Conference Paper uri icon

Overview

MeSH Major

  • Dystrophin
  • Sarcolemma

abstract

  • Serine proteases can induce cell signaling by stimulating G-protein-coupled receptors, called proteinase-activated receptors (PAR's) on a variety of epithelial cells. While PAR-2, one such receptor, activates cell signaling in a secretory cell line derived from human sweat glands, there was no information on their presence and effects on intact sweat glands. PAR-2 presence and activation of eccrine sweat glands isolated from human skin samples was investigated using Western blot analysis, immunohistochemistry, electron microscopy (EM) and Ca(2+) imaging. Anti-human PAR-2 antibody demonstrated the presence of these receptors in eccrine sweat glands. EM showed that PAR-2 activation resulted in degranulation of secretory cells. Ca(2+) imaging using PAR-2 activators demonstrated a two phase increase in [Ca(2+)](i) which was dependent on extracellular Ca(2+) for the second phase, and that the response could be blocked by prior incubation with xestospongin, the IP(3) receptor blocker. The results demonstrated that PAR-2 receptors are present in human sweat gland secretory cells and that these receptors are functionally active and can induce changes associated with secretory events in eccrine glands.

publication date

  • December 2009

Research

keywords

  • Conference Paper

Identity

Digital Object Identifier (DOI)

  • 10.2152/jmi.56.371

PubMed ID

  • 20224228

Additional Document Info

start page

  • 371

end page

  • 4

volume

  • 56

number

  • SUPPL. 1