Disruption of Paneth and goblet cell homeostasis and increased endoplasmic reticulum stress in Agr2-/- mice. Academic Article uri icon

Overview

MeSH

  • Animals
  • Inflammatory Bowel Diseases
  • Intestines
  • Mice
  • Mice, Knockout
  • Mucin-2
  • Stress, Physiological

MeSH Major

  • Endoplasmic Reticulum
  • Goblet Cells
  • Homeostasis
  • Mucoproteins
  • Paneth Cells

abstract

  • Anterior Gradient 2 (AGR2) is a protein disulfide isomerase that plays important roles in diverse processes in multiple cell lineages as a developmental regulator, survival factor and susceptibility gene for inflammatory bowel disease. Here, we show using germline and inducible Agr2-/- mice that Agr2 plays important roles in intestinal homeostasis. Agr2-/- intestine has decreased goblet cell Mucin 2, dramatic expansion of the Paneth cell compartment, abnormal Paneth cell localization, elevated endoplasmic reticulum (ER) stress, severe terminal ileitis and colitis. Cell culture experiments show that Agr2 expression is induced by ER stress, and that siRNA knockdown of Agr2 increases ER stress response. These studies implicate Agr2 in intestinal homeostasis and ER stress and suggest a role in the etiology of inflammatory bowel disease. Copyright 2009 Elsevier Inc. All rights reserved.

publication date

  • February 15, 2010

has subject area

  • Animals
  • Endoplasmic Reticulum
  • Goblet Cells
  • Homeostasis
  • Inflammatory Bowel Diseases
  • Intestines
  • Mice
  • Mice, Knockout
  • Mucin-2
  • Mucoproteins
  • Paneth Cells
  • Stress, Physiological

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2937056

Digital Object Identifier (DOI)

  • 10.1016/j.ydbio.2009.12.008

PubMed ID

  • 20025862

Additional Document Info

start page

  • 270

end page

  • 279

volume

  • 338

number

  • 2