Chemical screen to reduce sterol accumulation in Niemann-Pick C disease cells identifies novel lysosomal acid lipase inhibitors Academic Article uri icon

Overview

MeSH Major

  • Enzyme Inhibitors
  • Niemann-Pick Disease, Type C
  • Small Molecule Libraries
  • Sterol Esterase
  • Sterols

abstract

  • Niemann-Pick C disease (NPC) is a lysosomal storage disorder causing abnormal accumulation of unesterified free cholesterol in lysosomal storage organelles. High content phenotypic microscopy chemical screens in both human and hamster NPC-deficient cells have identified several compounds that partially revert the NPC phenotype. Cell biological and biochemical studies show that several of these molecules inhibit lysosomal acid lipase, the enzyme that hydrolyzes LDL-derived triacylglycerol and cholesteryl esters. The effects of reduced lysosomal acid lipase activity in lowering cholesterol accumulation in NPC mutant cells were verified by RNAi-mediated knockdown of lysosomal acid lipase in NPC1-deficient human fibroblasts. This work demonstrates the utility of phenotypic cellular screens as a means to identify molecular targets for altering a complex process such as intracellular cholesterol trafficking and metabolism.

publication date

  • September 17, 2009

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2783675

Digital Object Identifier (DOI)

  • 10.1016/j.bbalip.2009.08.005

PubMed ID

  • 19699313

Additional Document Info

start page

  • 1155

end page

  • 65

volume

  • 1791

number

  • 12