Clonally variant gene families in Plasmodium falciparum share a common activation factor. Academic Article uri icon

Overview

MeSH

  • Animals
  • Models, Biological
  • Protozoan Proteins

MeSH Major

  • Gene Expression Regulation
  • Genes, Protozoan
  • Plasmodium falciparum
  • Promoter Regions, Genetic
  • Transcriptional Activation

abstract

  • The genome of the malaria parasite Plasmodium falciparum contains several multicopy gene families, including var, rifin, stevor and Pfmc-2TM. These gene families undergo expression switching and appear to play a role in antigenic variation. It has recently been shown that forcing parasites to express high copy numbers of transcriptionally active, episomal var promoters led to gradual downregulation and eventual silencing of the entire var gene family, suggesting that a limiting titratable factor plays a role in var gene activation. Through similar experiments using rifin, stevor or Pfmc-2TM episomal promoters we show that promoter titration can be used as a general method to downregulate multicopy gene families in P. falciparum. Additionally, we show that promoter titration with var, rifin, stevor or Pfmc-2TM episomal promoters results in downregulation of expression not only of the family to which the episomal promoter belongs, but also members of the other gene families, suggesting that the var-specific titratable factor previously described is shared by all four families. Further, transcriptionally active promoters from different families colocalize within the same subnuclear expression site, indicating that the role that nuclear architecture plays in var gene regulation also likely applies to the other multicopy gene families of P. falciparum.

publication date

  • September 2009

has subject area

  • Animals
  • Gene Expression Regulation
  • Genes, Protozoan
  • Models, Biological
  • Plasmodium falciparum
  • Promoter Regions, Genetic
  • Protozoan Proteins
  • Transcriptional Activation

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2752644

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2958.2009.06846.x

PubMed ID

  • 19708920

Additional Document Info

start page

  • 1171

end page

  • 1185

volume

  • 73

number

  • 6