Influence of the cystic fibrosis transmembrane conductance regulator on expression of lipid metabolism-related genes in dendritic cells. Academic Article uri icon

Overview

MeSH

  • Animals
  • Bone Marrow Cells
  • Caveolin 1
  • Cystic Fibrosis
  • DNA Primers
  • Female
  • Genotype
  • Lung
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis
  • Pseudomonas aeruginosa
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sterol Regulatory Element Binding Proteins

MeSH Major

  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Dendritic Cells
  • Lipids

abstract

  • Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Infections of the respiratory tract are a hallmark in CF. The host immune responses in CF are not adequate to eradicate pathogens, such as P. aeruginosa. Dendritic cells (DC) are crucial in initiation and regulation of immune responses. Changes in DC function could contribute to abnormal immune responses on multiple levels. The role of DC in CF lung disease remains unknown. This study investigated the expression of CFTR gene in bone marrow-derived DC. We compared the differentiation and maturation profile of DC from CF and wild type (WT) mice. We analyzed the gene expression levels in DC from naive CF and WT mice or following P. aeruginosa infection. CFTR is expressed in DC with lower level compared to lung tissue. DC from CF mice showed a delayed in the early phase of differentiation. Gene expression analysis in DC generated from naive CF and WT mice revealed decreased expression of Caveolin-1 (Cav1), a membrane lipid raft protein, in the CF DC compared to WT DC. Consistently, protein and activity levels of the sterol regulatory element binding protein (SREBP), a negative regulator of Cav1 expression, were increased in CF DC. Following exposure to P. aeruginosa, expression of 3beta-hydroxysterol-Delta7 reductase (Dhcr7) and stearoyl-CoA desaturase 2 (Scd2), two enzymes involved in the lipid metabolism that are also regulated by SREBP, was less decreased in the CF DC compared to WT DC. These results suggest that CFTR dysfunction in DC affects factors involved in membrane structure and lipid-metabolism, which may contribute to the abnormal inflammatory and immune response characteristic of CF.

publication date

  • April 3, 2009

has subject area

  • Animals
  • Bone Marrow Cells
  • Caveolin 1
  • Cystic Fibrosis
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA Primers
  • Dendritic Cells
  • Female
  • Genotype
  • Lipids
  • Lung
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis
  • Pseudomonas aeruginosa
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sterol Regulatory Element Binding Proteins

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2683168

Digital Object Identifier (DOI)

  • 10.1186/1465-9921-10-26

PubMed ID

  • 19344509

Additional Document Info

start page

  • 26

volume

  • 10