A SNAIL1-SMAD3/4 transcriptional repressor complex promotes TGF-beta mediated epithelial-mesenchymal transition. Academic Article uri icon

Overview

MeSH

  • Animals
  • Blotting, Western
  • Cadherins
  • Cell Line, Transformed
  • Cell Nucleus
  • Chromatin Immunoprecipitation
  • Epithelial Cells
  • Humans
  • Intercellular Junctions
  • Mammary Neoplasms, Experimental
  • Mesoderm
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Fluorescence
  • Promoter Regions, Genetic
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
  • Snail Family Transcription Factors
  • Tumor Cells, Cultured

MeSH Major

  • Smad3 Protein
  • Smad4 Protein
  • Transcription Factors
  • Transforming Growth Factor beta

abstract

  • Epithelial-mesenchymal transition (EMT) is essential for organogenesis and is triggered during carcinoma progression to an invasive state. Transforming growth factor-beta (TGF-beta) cooperates with signalling pathways, such as Ras and Wnt, to induce EMT, but the molecular mechanisms are not clear. Here, we report that SMAD3 and SMAD4 interact and form a complex with SNAIL1, a transcriptional repressor and promoter of EMT. The SNAIL1-SMAD3/4 complex was targeted to the gene promoters of CAR, a tight-junction protein, and E-cadherin during TGF-beta-driven EMT in breast epithelial cells. SNAIL1 and SMAD3/4 acted as co-repressors of CAR, occludin, claudin-3 and E-cadherin promoters in transfected cells. Conversely, co-silencing of SNAIL1 and SMAD4 by siRNA inhibited repression of CAR and occludin during EMT. Moreover, loss of CAR and E-cadherin correlated with nuclear co-expression of SNAIL1 and SMAD3/4 in a mouse model of breast carcinoma and at the invasive fronts of human breast cancer. We propose that activation of a SNAIL1-SMAD3/4 transcriptional complex represents a mechanism of gene repression during EMT.

publication date

  • August 2009

has subject area

  • Animals
  • Blotting, Western
  • Cadherins
  • Cell Line, Transformed
  • Cell Nucleus
  • Chromatin Immunoprecipitation
  • Epithelial Cells
  • Humans
  • Intercellular Junctions
  • Mammary Neoplasms, Experimental
  • Mesoderm
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Fluorescence
  • Promoter Regions, Genetic
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
  • Smad3 Protein
  • Smad4 Protein
  • Snail Family Transcription Factors
  • Transcription Factors
  • Transforming Growth Factor beta
  • Tumor Cells, Cultured

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3769970

Digital Object Identifier (DOI)

  • 10.1038/ncb1905

PubMed ID

  • 19597490

Additional Document Info

start page

  • 943

end page

  • 950

volume

  • 11

number

  • 8