PET Imaging of Leptin Biodistribution and Metabolism in Rodents and Primates Academic Article uri icon

Overview

MeSH Major

  • Leptin

abstract

  • We have determined the systemic biodistribution of the hormone leptin by PET imaging. PET imaging using (18)F- and (68)Ga-labeled leptin revealed that, in mouse, the hormone was rapidly taken up by megalin (gp330/LRP2), a multiligand endocytic receptor localized in renal tubules. In addition, in rhesus monkeys, 15% of labeled leptin localized to red bone marrow, which was consistent with hormone uptake in rodent tissues. These data confirm a megalin-dependent mechanism for renal uptake in vivo. The significant binding to immune cells and blood cell precursors in bone marrow is also consistent with prior evidence showing that leptin modulates immune function. These experiments set the stage for similar studies in humans to assess the extent to which alterations of leptin's biodistribution might contribute to obesity; they also provide a general chemical strategy for (18)F labeling of proteins for PET imaging of other polypeptide hormones.

publication date

  • August 6, 2009

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2867490

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2009.07.001

PubMed ID

  • 19656493

Additional Document Info

start page

  • 148

end page

  • 59

volume

  • 10

number

  • 2