Evaluation of the clonal origin of multiple primary melanomas using molecular profiling Academic Article uri icon


MeSH Major

  • Melanoma
  • Mutation
  • Neoplasms, Multiple Primary
  • Skin Neoplasms


  • Numerous investigations have been conducted using molecular profiling to evaluate the possible clonal origin of second malignancies in various cancer types. However, to date no study assessing clonality of multiple primaries has been conducted in melanoma. In this investigation using patients treated at a specialist melanoma treatment center, we compared the somatic mutational profiles of pairs of melanomas designated as independent on the basis of thorough assessment of their clinical and pathologic characteristics. We used a set of highly polymorphic genetic markers selected on the basis of their chromosomal positions and the frequencies of reported allelic losses at these genetic loci. Our statistical testing strategy showed no significant evidence of clonal origin of the two primaries in 17 of the 19 patients examined. The results suggest that most second melanomas designated as independent primary tumors on the basis of their clinicopathologic features are indeed independent occurrences of the disease, supporting the validity of the criteria used by experienced pathologists in distinguishing new primaries from metastases.

publication date

  • August 2009



  • Academic Article



  • eng

PubMed Central ID

  • PMC2745834

Digital Object Identifier (DOI)

  • 10.1038/jid.2009.4

PubMed ID

  • 19282844

Additional Document Info

start page

  • 1972

end page

  • 82


  • 129


  • 8