Expression of B-cell activating factor enhances protective immunity of a vaccine against Pseudomonas aeruginosa. Academic Article uri icon

Overview

MeSH

  • Adenoviridae
  • Animals
  • Antibodies, Bacterial
  • Female
  • Genetic Vectors
  • Immunity, Mucosal
  • Mice
  • Mice, Inbred C57BL
  • Pneumonia, Bacterial
  • Survival Analysis
  • Vaccines, Inactivated

MeSH Major

  • Adjuvants, Immunologic
  • B-Cell Activating Factor
  • Pseudomonas Vaccines
  • Pseudomonas aeruginosa

abstract

  • B-cell activating factor (BAFF), a member of the TNF family, is a potent cytokine with stimulatory effects on B and T cells. To evaluate the potential of transient overexpression of BAFF to enhance vaccine immunogenicity, a replication-deficient adenovirus expressing full-length murine BAFF (AdBAFF) was tested in a mouse vaccine model against Pseudomonas aeruginosa. When coadministered with heat-killed P. aeruginosa, AdBAFF mediated a significant increase in anti-P. aeruginosa-specific serum and lung mucosal antibodies and resulted in improved protection against a lethal respiratory challenge with P. aeruginosa. This effect was independent of the site of administration of AdBAFF and was observed both when AdBAFF was given simultaneously with heat-killed P. aeruginosa as well as when AdBAFF was administered 4 weeks after immunization with heat-killed P. aeruginosa. These data demonstrate that a temporal increase in systemic BAFF levels is able to augment a P. aeruginosa-specific immune response upon immunization with heat-killed P. aeruginosa, suggesting that the immune-stimulatory effects of BAFF may be exploited as a molecular adjuvant for genetic vaccines.

publication date

  • July 2009

has subject area

  • Adenoviridae
  • Adjuvants, Immunologic
  • Animals
  • Antibodies, Bacterial
  • B-Cell Activating Factor
  • Female
  • Genetic Vectors
  • Immunity, Mucosal
  • Mice
  • Mice, Inbred C57BL
  • Pneumonia, Bacterial
  • Pseudomonas Vaccines
  • Pseudomonas aeruginosa
  • Survival Analysis
  • Vaccines, Inactivated

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2708570

Digital Object Identifier (DOI)

  • 10.1128/IAI.00927-08

PubMed ID

  • 19364838

Additional Document Info

start page

  • 3044

end page

  • 3055

volume

  • 77

number

  • 7