A nonradioactive dot blot assay for transglutaminase activity Academic Article uri icon

Overview

MeSH Major

  • Luminescent Measurements
  • Transglutaminases

abstract

  • Aberrant transglutaminase (TG) activity has been implicated in the pathology of numerous diseases, including Huntington's disease and Alzheimer's disease. To fully characterize the role of TGs in these disorders, it is important that simple quantifiable assays be made available. The most commonly used assay currently employed requires significant time and a radioactive substrate. The assay described here uses a biotinylated substrate in conjunction with a dot blot apparatus to eliminate the use of radioactive substrates and allows relative transglutaminase activity to be measured simultaneously with minimal sample preparation in a large number of samples containing purified enzyme, cell extracts, or tissue homogenates.

publication date

  • July 2009

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2845163

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2009.03.022

PubMed ID

  • 19306836

Additional Document Info

start page

  • 91

end page

  • 3

volume

  • 390

number

  • 1