Membrane antigen on Epstein--Barr virus-infected human B cells recognized by a monoclonal antibody. Academic Article uri icon

Overview

abstract

  • This paper describes a monoclonal antibody (B532) that detects a membrane antigen present on greater than or equal to 95% of the B cells from lines carrying the Epstein-Barr virus (EBV) genome. Evidence suggesting that B532 is EBV-related was originally obtained by using a cell-binding radioassay with different cell line substrates. Immunofluorescence and cell-sorter analysis confirmed that the antigen was present in high density on all EBV-infected lymphoblastoid B-cell lines, but not on EBV-negative B-, T-, myeloid, or null cell lines. Isolated normal peripheral blood B and T lymphocytes and monocytes failed to bind B532. The monoclonal antibody did not inhibit in vitro EVB infection nor did it block the killing of EBV-infected targets by cytotoxic T lymphocytes. The cell surface antigen recognized by B532 was shown by immunoprecipitation to have a molecular weight of approximately 45,000.

publication date

  • April 1, 1982

Research

keywords

  • Antibodies, Monoclonal
  • Antigens, Surface
  • B-Lymphocytes
  • Cell Transformation, Viral
  • Herpesvirus 4, Human

Identity

PubMed Central ID

  • PMC346258

Scopus Document Identifier

  • 0020315789

PubMed ID

  • 6283539

Additional Document Info

volume

  • 79

issue

  • 8