Phosphorylation of Gli2 by protein kinase A is required for Gli2 processing and degradation and the Sonic Hedgehog-regulated mouse development. Academic Article uri icon

Overview

MeSH

  • Animals
  • Body Patterning
  • Cell Death
  • Cell Proliferation
  • Mice
  • Mice, Mutant Strains
  • Motor Neurons
  • Mutation
  • Neural Tube
  • Phosphorylation
  • Signal Transduction
  • Spinal Cord

MeSH Major

  • Cyclic AMP-Dependent Protein Kinases
  • Hedgehog Proteins
  • Kruppel-Like Transcription Factors

abstract

  • In mice, Gli2 and Gli3 are the transcription factors that mediate the initial Hedgehog (Hh) signaling. In the absence of Hh signaling, the majority of the full-length Gli3 protein undergoes proteolytic processing into a repressor, while only a small fraction of the full-length Gli2 protein is processed. Gli3 processing is dependent on phosphorylation of the first four of the six protein kinase A (PKA) sites at its C-terminus. However, whether the same phosphorylation of Gli2 by PKA is required for Gli2 processing and, if so, whether such phosphorylation regulates additional Gli2 function are unknown. To address these questions, we mutated these PKA sites in the mouse Gli2 locus to create the Gli2(P1-4) allele. Gli2(P1-4) homozygous embryos die in utero and exhibit exencephaly, defects in neural tube closure, enlarged craniofacial structures, and an extra anterior digit. Analysis of spinal cord patterning shows that domains of motoneurons and V2, V1, and V0 interneurons are expanded to different degrees in both Gli2(P1-4) single and Gli2(P1-4);Shh double mutants. Furthermore, Gli2(P1-4) expression prevents massive cell death and promotes cell proliferation in Shh mutant. Analysis of Gli2(P1-4) protein in vivo reveals that the mutant protein is not processed and is twice as stable as wild type Gli2 protein. We also show that the Gli2 repressor can effectively antagonize Gli2P1-4 activity. Together, these results indicate that phosphorylation of Gli2 by PKA induces Gli2 processing and destabilization in vivo and plays an important role in the Hh-regulated mouse embryonic patterning.

publication date

  • February 1, 2009

has subject area

  • Animals
  • Body Patterning
  • Cell Death
  • Cell Proliferation
  • Cyclic AMP-Dependent Protein Kinases
  • Hedgehog Proteins
  • Kruppel-Like Transcription Factors
  • Mice
  • Mice, Mutant Strains
  • Motor Neurons
  • Mutation
  • Neural Tube
  • Phosphorylation
  • Signal Transduction
  • Spinal Cord

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2650378

Digital Object Identifier (DOI)

  • 10.1016/j.ydbio.2008.11.009

PubMed ID

  • 19056373

Additional Document Info

start page

  • 177

end page

  • 189

volume

  • 326

number

  • 1