Chromatin associated sense and antisense noncoding RNAs are transcribed from the var gene family of virulence genes of the malaria parasite Plasmodium falciparum. Academic Article uri icon

Overview

MeSH

  • Animals
  • Antigenic Variation
  • Base Sequence
  • Genes, Protozoan
  • In Situ Hybridization, Fluorescence
  • Introns
  • Molecular Sequence Data
  • Polyadenylation
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Virulence

MeSH Major

  • Chromatin
  • Plasmodium falciparum
  • Protozoan Proteins
  • RNA, Antisense
  • RNA, Protozoan
  • RNA, Untranslated

abstract

  • Antigenic variation by the malaria parasite Plasmodium falciparum results from switches in expression between members of the multicopy var gene family. These genes encode the variant surface protein PfEMP-1, the primary determinant of the antigenic and cytoadherent properties of infected erythrocytes. Only a single var gene is expressed at a time while the remaining members of the family remain transcriptionally silent. How mutually exclusive var gene expression is regulated is poorly understood; however, it is generally thought to involve alterations in chromatin assembly and modification, resulting in a type of cellular memory. Recently, several aspects of the chromatin structure surrounding var genes have been described, in particular the histone modifications associated with the active and silent states of the genes as well as their subnuclear localization. Here, we demonstrate that this chromatin structure also includes the incorporation of long sense and antisense noncoding RNAs. These sterile transcripts initiate from a bidirectional promoter located within a conserved intron found in all var genes that was previously implicated in var gene silencing. Mapping of the 5' and 3' ends of the sterile transcripts indicates that they are nonpolyadenylated. RNA fluorescent in situ hybridization (RNA-FISH) analysis detects both the sense and antisense noncoding RNAs in distinct spots within the nucleus similar to the pattern described for the var genes themselves. Further, analysis by RNA chromatin immunoprecipitation (ChIP) indicates that the noncoding RNAs are physically associated with chromatin. These sterile transcripts therefore might act in a manner analogous to noncoding RNAs associated with silent, condensed chromatin found in other eukaryotic systems.

publication date

  • January 2009

has subject area

  • Animals
  • Antigenic Variation
  • Base Sequence
  • Chromatin
  • Genes, Protozoan
  • In Situ Hybridization, Fluorescence
  • Introns
  • Molecular Sequence Data
  • Plasmodium falciparum
  • Polyadenylation
  • Promoter Regions, Genetic
  • Protozoan Proteins
  • RNA, Antisense
  • RNA, Protozoan
  • RNA, Untranslated
  • Transcription, Genetic
  • Virulence

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2612763

Digital Object Identifier (DOI)

  • 10.1261/rna.1080109

PubMed ID

  • 19037012

Additional Document Info

start page

  • 116

end page

  • 127

volume

  • 15

number

  • 1