Overexpression of apoptotic cell removal receptor MERTK in alveolar macrophages of cigarette smokers. Academic Article uri icon

Overview

MeSH

  • Adult
  • Animals
  • Blotting, Western
  • Bronchoalveolar Lavage Fluid
  • Cell Line
  • Female
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Male
  • Phagocytosis
  • Proto-Oncogene Proteins
  • Pulmonary Disease, Chronic Obstructive
  • Rats
  • Receptor Protein-Tyrosine Kinases
  • Receptors, Cell Surface
  • Solubility
  • Up-Regulation

MeSH Major

  • Apoptosis
  • Macrophages, Alveolar
  • Smoking

abstract

  • Mononuclear phagocytes play an important role in the removal of apoptotic cells by expressing cell surface receptors that recognize and remove apoptotic cells. Based on the knowledge that cigarette smoking is associated with increased lung cell turnover, we hypothesized that alveolar macrophages (AMs) of normal cigarette smokers may exhibit enhanced expression of apoptotic cell removal receptor genes. AMs obtained by bronchoalveolar lavage of normal nonsmokers (n = 11) and phenotypic normal smokers (n = 13; 36 +/- 6 pack-years) were screened for mRNA expression of all known apoptotic cell removal receptors using Affymetrix HG-U133 Plus 2.0 microarray chips with TaqMan RT-PCR confirmation. Of the 14 known apoptotic receptors expressed, only MER tyrosine kinase (MERTK), a transmembrane tyrosine kinase receptor, was significantly up-regulated in smokers. MERTK expression was then assessed in AMs of smokers versus nonsmokers by TaqMan RT-PCR, immunocytochemistry, Western analysis, and flow analysis. Smoker AMs had up-regulation of MERTK mRNA levels (smoker vs. nonsmoker: 3.6-fold by microarray, P < 0.003; 9.5-fold by TaqMan RT-PCR, P < 0.02). Immunocytochemistry demonstrated a qualitative increase in MERTK protein expression on AMs of smokers. Increased protein expression of MERTK on AMs of smokers was confirmed by Western and flow analyses (P < 0.007 and P < 0.0002, respectively). MERTK, a cell surface receptor that recognizes apoptotic cells, is expressed on human AMs, and its expression is up-regulated in AMs of cigarette smokers. This up-regulation of MERTK may reflect an increased demand for removal of apoptotic cells in smokers, an observation with implications for the development of chronic obstructive pulmonary disease, a disorder associated with dysregulated apoptosis of lung parenchymal cells.

publication date

  • December 2008

has subject area

  • Adult
  • Animals
  • Apoptosis
  • Blotting, Western
  • Bronchoalveolar Lavage Fluid
  • Cell Line
  • Female
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Macrophages, Alveolar
  • Male
  • Phagocytosis
  • Proto-Oncogene Proteins
  • Pulmonary Disease, Chronic Obstructive
  • Rats
  • Receptor Protein-Tyrosine Kinases
  • Receptors, Cell Surface
  • Smoking
  • Solubility
  • Up-Regulation

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2586050

Digital Object Identifier (DOI)

  • 10.1165/rcmb.2007-0306OC

PubMed ID

  • 18587056

Additional Document Info

start page

  • 747

end page

  • 757

volume

  • 39

number

  • 6