Automatic tracking of proteins in sequences of fluorescence images Conference Paper uri icon

Overview

MeSH Major

  • Macular Edema
  • Retinal Vein Occlusion

abstract

  • The development of digital microscopy and computational power is providing new opportunities for analyzing the motility of the vesicles (proteins) within living cells. In this paper, an automatic method is developed to segment and track vesicles in large amount of fluorescence images, in order to compute a number of quantitative parameters such as displacement, residence time, binding, or immobile fraction. We present a method that permits the automatic tracking of subcellular structures in long sequences of fluorescence images (up to 100 frames). The method we propose has been tested on 92 data sets totaling 8225 frames.

publication date

  • October 27, 2004

Research

keywords

  • Conference Paper

Identity

Digital Object Identifier (DOI)

  • 10.1117/12.533726

Additional Document Info

start page

  • 1364

end page

  • 1371

volume

  • 5370 II