Induced pluripotent stem cells generated without viral integration Academic Article Article uri icon

Overview

MeSH Major

  • Calcitriol
  • Parathyroid Hormone
  • Receptors, Calcitriol
  • Transcription, Genetic

abstract

  • Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc. A major limitation of this technology is the use of potentially harmful genome-integrating viruses. We generated mouse induced pluripotent stem (iPS) cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc. These adenoviral iPS (adeno-iPS) cells show DNA demethylation characteristic of reprogrammed cells, express endogenous pluripotency genes, form teratomas, and contribute to multiple tissues, including the germ line, in chimeric mice. Our results provide strong evidence that insertional mutagenesis is not required for in vitro reprogramming. Adenoviral reprogramming may provide an improved method for generating and studying patient-specific stem cells and for comparing embryonic stem cells and iPS cells.

publication date

  • November 7, 2008

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1126/science.1162494

PubMed ID

  • 18818365

Additional Document Info

start page

  • 945

end page

  • 949

volume

  • 322

number

  • 5903